diff --git a/app/Config/Routes.php b/app/Config/Routes.php
index c45a065..616728e 100644
--- a/app/Config/Routes.php
+++ b/app/Config/Routes.php
@@ -52,6 +52,7 @@ $routes->get('(:any)/audit', 'ApiRequestsAuditController::show/$1');
         // Collect & Show - All Roles
         $routes->group('', ['filter' => 'role:0,1,2,3,4'], function ($routes) {
             $routes->post('collect/(:any)/(:any)', 'SamplesController::collect/$1/$2');
+            $routes->delete('collect/(:any)/(:any)', 'SamplesController::uncollect/$1/$2');
             $routes->get('(:any)', 'SamplesController::show/$1');
         });
 
diff --git a/app/Controllers/SamplesController.php b/app/Controllers/SamplesController.php
index b1f8b1b..691c27d 100644
--- a/app/Controllers/SamplesController.php
+++ b/app/Controllers/SamplesController.php
@@ -68,14 +68,26 @@ class SamplesController extends BaseController
   {
     $db = \Config\Database::connect();
     $userid = session('userid');
-    $sql = "update GDC_CMOD.dbo.TUBES set USERID='$userid',STATUS='1', COLLECTIONDATE=getdate() where ACCESSNUMBER='$accessnumber' and TUBENUMBER='$samplenumber'";
-    $db->query($sql);
+    $sql = "update GDC_CMOD.dbo.TUBES set USERID=?, STATUS='1', COLLECTIONDATE=getdate() where ACCESSNUMBER=? and TUBENUMBER=?";
+    $db->query($sql, [$userid, $accessnumber, $samplenumber]);
     $sql = "INSERT INTO GDC_CMOD.dbo.AUDIT_TUBES(ACCESSNUMBER, TUBENUMBER, USERID, STATUS, LOGDATE)
-      VALUES ('$accessnumber', '$samplenumber', '$userid', '1', getdate())";
-    $db->query($sql);
+      VALUES (?, ?, ?, '1', getdate())";
+    $db->query($sql, [$accessnumber, $samplenumber, $userid]);
     return $this->respondCreated(['status' => 'success', 'message' => 'Data updated successfully', 'data' => "$accessnumber-$samplenumber"], 201);
   }
 
+  public function uncollect($accessnumber, $samplenumber)
+  {
+    $db = \Config\Database::connect();
+    $userid = session('userid');
+    $sql = "update GDC_CMOD.dbo.TUBES set STATUS='0', COLLECTIONDATE=NULL where ACCESSNUMBER=? and TUBENUMBER=?";
+    $db->query($sql, [$accessnumber, $samplenumber]);
+    $sql = "INSERT INTO GDC_CMOD.dbo.AUDIT_TUBES(ACCESSNUMBER, TUBENUMBER, USERID, STATUS, LOGDATE)
+      VALUES (?, ?, ?, '0', getdate())";
+    $db->query($sql, [$accessnumber, $samplenumber, $userid]);
+    return $this->respond(['status' => 'success', 'message' => 'Sample uncollected successfully', 'data' => "$accessnumber-$samplenumber"], 200);
+  }
+
   public function unreceive($accessnumber, $samplenumber)
   {
     $db = \Config\Database::connect();
diff --git a/app/Views/phlebo/collect.php b/app/Views/phlebo/collect.php
index bae183d..5ab341f 100644
--- a/app/Views/phlebo/collect.php
+++ b/app/Views/phlebo/collect.php
@@ -100,13 +100,11 @@ $roleConfig = $config['phlebo'];
                             </thead>
                             <tbody>
                                 <template x-for="sample in samples" :key="sample.sampcode">
-                                    <tr :class="sample.colstatus == 1 ? 'bg-success/10' : (sample.selected ? 'bg-warning/10' : '')">
+                                     <tr :class="sample.selected ? (sample.colstatus == 1 ? 'bg-success/10' : 'bg-warning/10') : ''">
                                         <td class="text-center p-2">
                                             <input type="checkbox" 
                                                    class="checkbox checkbox-xs checkbox-primary"
-                                                   x-model="sample.selected"
-                                                   :checked="sample.colstatus == 1"
-                                                   :disabled="sample.colstatus == 1">
+                                                   x-model="sample.selected">
                                         </td>
                                         <td class="font-mono text-xs p-2" x-text="sample.sampcode"></td>
                                         <td class="text-xs p-2" x-text="sample.name"></td>
@@ -141,7 +139,7 @@ $roleConfig = $config['phlebo'];
                             </button>
                             <button class="btn btn-sm btn-primary" 
                                     @click="saveCollection()" 
-                                    :disabled="isSaving || !hasSelectedSamples()"
+                                    :disabled="isSaving || !hasChanges()"
                                     :class="isSaving ? 'loading' : ''">
                                 <i class="fa fa-save mr-1" x-show="!isSaving"></i>
                                 <span x-text="isSaving ? 'Saving' : 'Save'"></span>
@@ -227,6 +225,12 @@ $roleConfig = $config['phlebo'];
                 return this.samples.some(s => s.selected && s.colstatus != 1);
             },
 
+            hasChanges() {
+                const toCollect = this.samples.some(s => s.selected && s.colstatus != 1);
+                const toUncollect = this.samples.some(s => !s.selected && s.colstatus == 1);
+                return toCollect || toUncollect;
+            },
+
             hasUncollectedSamples() {
                 return this.samples.some(s => s.colstatus != 1 && !s.selected);
             },
@@ -271,15 +275,17 @@ $roleConfig = $config['phlebo'];
                 
                 try {
                     const samplesToCollect = this.samples.filter(s => s.selected && s.colstatus != 1);
+                    const samplesToUncollect = this.samples.filter(s => !s.selected && s.colstatus == 1);
                     
                     for (const sample of samplesToCollect) {
-                        await fetch(`${BASEURL}/api/samples/collect/${this.patient.accessnumber}`, {
-                            method: 'POST',
-                            headers: { 'Content-Type': 'application/json' },
-                            body: JSON.stringify({
-                                samplenumber: sample.sampcode,
-                                userid: this.userid
-                            })
+                        await fetch(`${BASEURL}/api/samples/collect/${this.patient.accessnumber}/${sample.sampcode}`, {
+                            method: 'POST'
+                        });
+                    }
+                    
+                    for (const sample of samplesToUncollect) {
+                        await fetch(`${BASEURL}/api/samples/collect/${this.patient.accessnumber}/${sample.sampcode}`, {
+                            method: 'DELETE'
                         });
                     }
 
@@ -287,10 +293,11 @@ $roleConfig = $config['phlebo'];
                         await this.saveComment();
                     }
 
-                    this.showToast(`Collected ${samplesToCollect.length} sample(s)`, 'success');
+                    const totalChanged = samplesToCollect.length + samplesToUncollect.length;
+                    this.showToast(`Updated ${totalChanged} sample(s)`, 'success');
                     
                     setTimeout(() => {
-                        this.resetForm();
+                        this.fetchPatientData();
                     }, 1000);
                 } catch (error) {
                     console.error('Error:', error);